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Each organ of this animal has already been investigated by the method of using a fluorescent imaging system, but not by histological methods. First of all, we compared all the organs of the red fluorescent transgenic mouse with previous studies using enhanced green fluorescent protein (EGFP) mice by the IVIS Luminal Imaging. Next the expression levels of each organs mRNA was measured by RT-PCR. Further, the histological sections of the red fluorescent transgenic mice (7 days after-birth) were taken by light microscope. The results of the three methods were compared and the following findings were made ; We found a discrepancy between the fluorescent imaging method which was used in past reports and the expression levels of mRNA and multiple discrepancies between these and the histological observations. Explaining the discrepancies was beyond the scope of this experiment. We thought that two or more methods including the histological examination were reguired by future research and verification. The red fluorescent transgenic mouse was more useful than the EGFP mouse in the fluorescent expression of the hepar, spleen and ren. All of the organs in the red fluorescent transgenic mouse have a strong fluorescent expression. We hope that these red fluorescent transgenic mice are used in the research of regenerative medicine and the metastic route of the tumor stem cell.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Division of Orthodontics, Department of Developmental Oral Health Science, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Basic Molecular Science and Molecular Medicine, Department of Molecular Life Science, School of Medicine, Tokai University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Functional Morphology, Department of Anatomy, Iwate Medical University"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔保健育成学講座歯科矯正学分野"}, {"subitem_text_value": "東海大学医学部基礎医学系分子生命科学"}, {"subitem_text_value": "岩手医科大学生化学講座細胞情報科学分野"}, {"subitem_text_value": "岩手医科大学解剖学講座機能形態学分野"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2012-05-11"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00008045759.pdf", "filesize": [{"value": "36.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 36400000.0, "url": {"label": "KJ00008045759", "url": "https://iwatemed.repo.nii.ac.jp/record/2396/files/KJ00008045759.pdf"}, "version_id": "598fe998-dfc0-4ae8-9eef-cc3f0bbf2ad5"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "dTomato", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transgenic mouse", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "fluoresce microscopy", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "EGFP", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討"}, {"subitem_title": "Anatomical analyses on the expression of the fluorescence in tdTomato transgenic mice", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["116", "124", "126", "560"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/2396", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-05-11"}, "publish_date": "2012-05-11", "publish_status": "0", "recid": "2396", "relation": {}, "relation_version_is_last": true, "title": ["赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討"], "weko_shared_id": 5}
赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討
https://iwatemed.repo.nii.ac.jp/records/2396
https://iwatemed.repo.nii.ac.jp/records/23964183bba6-4321-4844-a59d-6763a0b8d5c7
名前 / ファイル | ライセンス | アクション |
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KJ00008045759 (36.4 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-05-11 | |||||
タイトル | ||||||
タイトル | 赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Anatomical analyses on the expression of the fluorescence in tdTomato transgenic mice | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者名(日) |
桑島, 幸紀
× 桑島, 幸紀× 大塚, 正人× 石崎, 明× 藤村, 朗 |
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著者名よみ |
クワジマ, ユキノリ
× クワジマ, ユキノリ× オオツカ, マサト× イシザキ, アキラ× フジムラ, アキラ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 37, 号 1, p. 24-37, 発行日 2012-05-11 |