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Here we investigated the role of Rab37, one of the Rab GTPases assumed to be implicated in regulated secretion during mast cell degranulation. Using the RBL-2H3 mast cell line, we detected Rab37 on the secretory granules and found that antigen-induced degranulation was extensively increased by either knockdown of Rab37 or overexpression of a dominant-active Rab37 mutant. This hypersecretion phenotype in the Rab37-knockdown cells was suppressed by simultaneous knockdown of Rab27a and Rab27b or of Munc13-4, but not by disruption of cortical F-actin. We further found that Rab37 interacted with Munc13-4 in a GTP-independent manner and formed a Rab27-Munc13-4-Rab37 complex. These results suggest that Rab37 is a Munc13-4-binding protein that inhibits mast cell degranulation through its effector protein, by counteracting the vesicle-priming activity of the Rab27-Munc13-4 system.", "subitem_description_type": "Abstract"}]}, "item_10001_description_6": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Mast cell degranulation is regulated by the small guanosine triphosphatases (GTPases) Rab27a and Rab27b, which have distinct and opposing roles: Rab27b acts as a positive regulator through its effector protein Munc13-4, a non-neuronal isoform of the vesicle-priming Munc13 family of proteins, whereas Rab27a acts as a negative regulator through its effector protein melanophilin, by maintaining integrity of cortical filamentous actin (F-actin), a barrier to degranulation. Here we investigated the role of Rab37, one of the Rab GTPases assumed to be implicated in regulated secretion during mast cell degranulation. Using the RBL-2H3 mast cell line, we detected Rab37 on the secretory granules and found that antigen-induced degranulation was extensively increased by either knockdown of Rab37 or overexpression of a dominant-active Rab37 mutant. This hypersecretion phenotype in the Rab37-knockdown cells was suppressed by simultaneous knockdown of Rab27a and Rab27b or of Munc13-4, but not by disruption of cortical F-actin. We further found that Rab37 interacted with Munc13-4 in a GTP-independent manner and formed a Rab27-Munc13-4-Rab37 complex. These results suggest that Rab37 is a Munc13-4-binding protein that inhibits mast cell degranulation through its effector protein, by counteracting the vesicle-priming activity of the Rab27-Munc13-4 system.", "subitem_description_type": "Other"}]}, "item_10001_heading_23": {"attribute_name": "見出し", "attribute_value_mlt": [{"subitem_heading_banner_headline": "教養教育センター", "subitem_heading_headline": "化学", "subitem_heading_language": "ja"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nature Publishing Group"}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "26931073", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1038/srep22539", "subitem_relation_type_select": "DOI"}}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "東尾, 浩典"}, {"creatorName": "ひがしお, ひろのり", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "21368", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higashio, Hironori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21365", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Satoh, Yoh-Ichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21366", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saino, Tomoyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "21367", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "B02201603HH.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "本文", "url": "https://iwatemed.repo.nii.ac.jp/record/5719/files/B02201603HH.pdf"}, "version_id": "5c993db9-91a0-4260-9a97-bbd75dc713d9"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "B02201603HHs.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "Supplementary information", "url": "https://iwatemed.repo.nii.ac.jp/record/5719/files/B02201603HHs.pdf"}, "version_id": "a9283443-200f-400d-b492-feda20c6eecd"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37."}, {"subitem_title": "Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37.", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "27", "path": ["79", "150"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/5719", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-04-21"}, "publish_date": "2016-04-21", "publish_status": "0", "recid": "5719", "relation": {}, "relation_version_is_last": true, "title": ["Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37."], "weko_shared_id": -1}
Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37.
https://iwatemed.repo.nii.ac.jp/records/5719
https://iwatemed.repo.nii.ac.jp/records/571950bda671-267c-4827-80a9-f161b18dd0a4
名前 / ファイル | ライセンス | アクション |
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本文 (1.7 MB)
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Supplementary information (2.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-04-21 | |||||
タイトル | ||||||
タイトル | Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37. | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Mast cell degranulation is negatively regulated by the Munc13-4-binding small-guanosine triphosphatase Rab37. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
東尾, 浩典
× 東尾, 浩典× Higashio, Hironori× Satoh, Yoh-Ichi× Saino, Tomoyuki |
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書誌情報 |
Scientific reports en : Scientific reports 巻 6, p. 22539, 発行日 2016-03-02 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 26931073 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/srep22539 | |||||
見出し | ||||||
大見出し | 教養教育センター | |||||
小見出し | 化学 | |||||
言語 | ja |