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In the present study, we examined whether sex hormones as shown by the analysis of hormonel level in CKS have the capacity to induce lipogenesis in Meckel\u0027s chondrocytes in vitro. Cultures were treated with different sex hormones at physiological and pharmacological concentrations, including testosterone and 17β-estradiol (E_2). Lipogenesis was evaluated by sudan staining. Testosterone and E_2, which were the two most effective hormones, were selected for the present study and their effects were further examined by histological and immunohistochemical methods, including immunolocalization of adipocyte markers. BrdU-incorporation in cells exposed to physiological concentration of E_2 and testosterone was increased compared to control cultures, but there was no significant difference in BrdU-incorporation in cultures treated with E_2 and testosterone. However, at pharmacological concentrations, these hormones induced dose-dependent lipogenesis in Meckel\u0027s chondrocytes. Almost all the lipid droplet-containing cells were positive for leptin, α-glycerophosphate dehydrogenase (GPDH), and CCAAT-enhancer binding protein (C/EBPα), as shown by immunoperoxidase staining. Testosterone and E_2 had no capacity to induce lipogenesis in Meckel\u0027s chondrocytes at physiological concentrations, but our results suggest that they have the potential for lipogenesis as a pharmacological effect.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Dental Radiology, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II Iwate Medical University"}]}, "item_2_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "研究"}]}, "item_2_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Original"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2005-04-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00002592206.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "KJ00002592206", "url": "https://iwatemed.repo.nii.ac.jp/record/1270/files/KJ00002592206.pdf"}, "version_id": "57a12f3a-e886-4b86-b2c8-9b2becbcfa64"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "メッケル軟骨細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "脂肪形成", "subitem_subject_scheme": "Other"}, {"subitem_subject": "性ホルモン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞培養", "subitem_subject_scheme": "Other"}, {"subitem_subject": "免疫組織化学", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Meckel\u0027s chondrocytes", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lipogenesis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sex hormones", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cell culture", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "immunohistochemistry", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "培養メッケル軟骨細胞に対する性ホルモンの脂肪形成効果について", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "培養メッケル軟骨細胞に対する性ホルモンの脂肪形成効果について"}, {"subitem_title": "Pharmacological effects of sex steroid hormones on lipogenesis in Meckel\u0027s chondrocytes in vitro", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["125", "378"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/1270", "pubdate": {"attribute_name": "公開日", "attribute_value": "2005-04-25"}, "publish_date": "2005-04-25", "publish_status": "0", "recid": "1270", "relation": {}, "relation_version_is_last": true, "title": ["培養メッケル軟骨細胞に対する性ホルモンの脂肪形成効果について"], "weko_shared_id": 5}
培養メッケル軟骨細胞に対する性ホルモンの脂肪形成効果について
https://iwatemed.repo.nii.ac.jp/records/1270
https://iwatemed.repo.nii.ac.jp/records/12705769e62e-ae2c-4014-a390-5e572ecc75ff
名前 / ファイル | ライセンス | アクション |
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KJ00002592206 (1.8 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2005-04-25 | |||||
タイトル | ||||||
タイトル | 培養メッケル軟骨細胞に対する性ホルモンの脂肪形成効果について | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Pharmacological effects of sex steroid hormones on lipogenesis in Meckel's chondrocytes in vitro | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
石関, 清人
× 石関, 清人× 高橋, 徳明× 名和, 橙黄雄 |
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著者名よみ |
イシゼキ, キヨト
× イシゼキ, キヨト× タカハシ, ノリアキ× ナワ, トキオ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 30, 号 1, p. 42-52, 発行日 2005-04-25 |