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Specimens for cell and organ cultures were cultured for 4 weeks in different media as follows: Control medium, alpha-modified Eagle\u0027s medium (α-MEM); β-Gly medium, α-MEM plus β-glycerophosphate (β-Gly); ELC medium,α-MEM plus ELC; and β-Gly \u0026 ELC medium,α-MEM plus β-Gly plus ELC. Specimens were analyzed by histological and histochemical methods, including immunohistochemistry for alkaline phosphatase (ALPase) activity and bromodeoxyuridine (BrdU) incorporation. Von Kossa staining revealed that the ELC medium inducedeffective calcification similar to β-Gly medium, while BrdU incorporation showed that there was no significant difference in the rates of cell proliferation between Control medium and ELC medium. Histochemical and immunofluorescence staining indicated that ALPase was activated prior to matrix calcification in cell and organ cultures. β-Gly \u0026 ELC medium induced synergistically the highest level of matrix calcification and ALPase activity. The present results suggest the possibility that ELC accelerates activity of ALPase in Meckel\u0027s chondrocytes and subsequently induces matrix calcification.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy, School of Dentistry, Iwate Medical University"}]}, "item_2_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "研究"}]}, "item_2_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Original"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2008-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00005015086.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "KJ00005015086", "url": "https://iwatemed.repo.nii.ac.jp/record/1554/files/KJ00005015086.pdf"}, "version_id": "d46d369a-d125-4532-a6d4-6df00e9e5a56"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "メッケル軟骨", "subitem_subject_scheme": "Other"}, {"subitem_subject": "軟骨細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "エルカトニン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "石灰化", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞培養と器官培養", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Meckel\u0027s cartilage", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chondrocyte", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Elcatonin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Calcification", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cell and organ culture", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "培養メッケル軟骨の石灰化に対するエルカトニンの効果", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "培養メッケル軟骨の石灰化に対するエルカトニンの効果"}, {"subitem_title": "Effects of elcatonin on matrix calcification of Meckel\u0027s cartilage in vitro", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["125", "391"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/1554", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-08-20"}, "publish_date": "2008-08-20", "publish_status": "0", "recid": "1554", "relation": {}, "relation_version_is_last": true, "title": ["培養メッケル軟骨の石灰化に対するエルカトニンの効果"], "weko_shared_id": 5}
培養メッケル軟骨の石灰化に対するエルカトニンの効果
https://iwatemed.repo.nii.ac.jp/records/1554
https://iwatemed.repo.nii.ac.jp/records/1554e3c7568a-6baf-44ac-8db6-afa963b7c454
名前 / ファイル | ライセンス | アクション |
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KJ00005015086 (1.9 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-08-20 | |||||
タイトル | ||||||
タイトル | 培養メッケル軟骨の石灰化に対するエルカトニンの効果 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effects of elcatonin on matrix calcification of Meckel's cartilage in vitro | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
石関, 清人
× 石関, 清人× 鍵谷, 忠慶× 藤原, 尚樹× 原田, 英光 |
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著者名よみ |
イシゼキ, キヨト
× イシゼキ, キヨト× カギヤ, タダヨシ× フジワラ, ナオキ× ハラダ, ヒデミツ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 33, 号 2, p. 58-69, 発行日 2008-08-20 |