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In the present study, CKS-induced adipogenesis on the osteogenic MC3T3-E1 cells, mouse subcutaneous fibroblasts (MSF), and undifferentiated mouse dental pulp cells (MDP) were examined using light and electron microscopy, immunofluorescence staining, and BrdU-incorporation for DNA-synthesizing cells. Furthermore, MSF was cultured on a collagen-gel matrix that allowed maintenance of three-dimensional integrity. CKS facilitated lipogenesis and induced the formation of lipid droplets in all kinds of cells. Immunostaining showed the expression of peroxisome proliferator-activated receptor-γ in immature adipocytes, and leptin and glucose transporter-4 in mature adipocytes. Adipocytes on collagen-gel matrix induced unilocular lipid droplets at a high frequency. These morphological observations strongly indicate that these lipid-rich cells retain sufficient features as intact adipocytes, although the adipocytes that were formed in the present study were multilocular adipocytes. 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種々の間葉系細胞における脂肪細胞の分化誘導について : ニワトリ血清因子の効果
https://iwatemed.repo.nii.ac.jp/records/1650
https://iwatemed.repo.nii.ac.jp/records/1650c5cf09d1-c9b8-4afb-86ca-e0165b08fe29
名前 / ファイル | ライセンス | アクション |
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KJ00002373613 (1.2 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2002-12-24 | |||||
タイトル | ||||||
タイトル | 種々の間葉系細胞における脂肪細胞の分化誘導について : ニワトリ血清因子の効果 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effects of chick serum agents on the induction of adipocyte differentiation in various mesenchymal cells | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
高橋, 徳明
× 高橋, 徳明 |
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著者名よみ |
タカハシ, ノリアキ
× タカハシ, ノリアキ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 27, 号 3, p. 166-177, 発行日 2002-12-24 |