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Cell death was induced in vitro in Meckel\u0027s chondrocytes that were enzymatically isolated from 16-day gestation mouse embryos. The effects were assayed histologically and immunohistochemically by using the TUNEL procedure, and light and electron microscopy. Etoposide and camptothecin-treated cultures were followed by the strong appearance of TUNEL (TdT-mediated biotinylated dUTP nick end-labeling)-positive apoptotic cells; statistical analysis showed that these agents induced apoptosis significantly 12-24hrs after treatment. At an ultrastructural level, apoptotic cell death from etoposide was followed by the formation of cell blebs, chromatin condensation and the formation of apoptotic bodies. Immunostaining for p53 revealed that this protein was absent from intact chondrocytes but was continuously accelerated in the cells treated with etoposide and camptothecin. Bcl- 2 was immunolocalized in intact chondrocytes at an early stage of culture, but was not detected in anticancer-treated cells. In the present study, we confirmed that cell death in Meckel\u0027s chondrocytes induced by etoposide and camptothecin is typical apoptotic cell death, and that these agents also induce apoptosis in intact chondrocytes.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Anatomy II, School of Dentistry, Iwate Medical University"}]}, "item_2_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "研究"}]}, "item_2_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Originals"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}, {"subitem_text_value": "岩手医科大学歯学部口腔解剖学第二講座"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2003-12-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00002373709.pdf", "filesize": [{"value": "3.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 3100000.0, "url": {"label": "KJ00002373709", "url": "https://iwatemed.repo.nii.ac.jp/record/1746/files/KJ00002373709.pdf"}, "version_id": "90ceffec-e7b1-4955-8131-f51a6037ec14"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "メッケル軟骨", "subitem_subject_scheme": "Other"}, {"subitem_subject": "軟骨細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "アポトーシス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "抗癌剤", "subitem_subject_scheme": "Other"}, {"subitem_subject": "エトポシトとカンプトセンン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Meckel\u0027s cartilage", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chondrocyte", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Apoptosis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Anticancer agent", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Etoposide and camptothecin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "抗癌剤によって培養メッケル軟骨に誘導されるアポトーシス細胞の特徴", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "抗癌剤によって培養メッケル軟骨に誘導されるアポトーシス細胞の特徴"}, {"subitem_title": "Characterization of apoptotic cells induced in vitro in Meckel\u0027s chondrocytes by anticancer agents", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["125", "401"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/1746", "pubdate": {"attribute_name": "公開日", "attribute_value": "2003-12-24"}, "publish_date": "2003-12-24", "publish_status": "0", "recid": "1746", "relation": {}, "relation_version_is_last": true, "title": ["抗癌剤によって培養メッケル軟骨に誘導されるアポトーシス細胞の特徴"], "weko_shared_id": 5}
抗癌剤によって培養メッケル軟骨に誘導されるアポトーシス細胞の特徴
https://iwatemed.repo.nii.ac.jp/records/1746
https://iwatemed.repo.nii.ac.jp/records/174609122577-4211-4ef3-9aed-b1ed3a1b1dc9
名前 / ファイル | ライセンス | アクション |
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KJ00002373709 (3.1 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2003-12-24 | |||||
タイトル | ||||||
タイトル | 抗癌剤によって培養メッケル軟骨に誘導されるアポトーシス細胞の特徴 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterization of apoptotic cells induced in vitro in Meckel's chondrocytes by anticancer agents | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Originals | ||||||
著者名(日) |
品川, 拓人
× 品川, 拓人× 原田, 順男× 熊上, 亮× 石関, 清人 |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 28, 号 3, p. 158-170, 発行日 2003-12-24 |