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{"_buckets": {"deposit": "3193f7f9-1186-4136-bfa0-2a7c643c2b41"}, "_deposit": {"created_by": 5, "id": "2006", "owners": [5], "pid": {"revision_id": 0, "type": "depid", "value": "2006"}, "status": "published"}, "_oai": {"id": "oai:iwatemed.repo.nii.ac.jp:00002006", "sets": ["119", "483"]}, "author_link": ["7003", "7005", "7001", "7002", "7006", "7004"], "item_2_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-05-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "18", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "36", "bibliographic_titles": [{"bibliographic_title": "岩手医科大学歯学雑誌"}, {"bibliographic_title": "Dental journal of Iwate Medical University", "bibliographic_titleLang": "en"}]}]}, "item_2_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "羽田, 朋弘"}], "nameIdentifiers": [{"nameIdentifier": "7001", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "三上, 俊成"}], "nameIdentifiers": [{"nameIdentifier": "7002", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_creator_7": {"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ハダ, トモヒロ"}], "nameIdentifiers": [{"nameIdentifier": "7003", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ミカミ, トシナリ"}], "nameIdentifiers": [{"nameIdentifier": "7004", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_creator_8": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "HADA, Tomohiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIKAMI, Toshinari", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7006", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "多形腺腫は唾液腺おいて最も高頻度に発生する良性の上皮性腫瘍である.導管上皮由来の細胞と,筋上皮細胞由来の細胞が二相性に増殖していることが病理学的特徴である.WT1遺伝子は小児腎腫瘍であるWilms腫瘍の原因遺伝子として単離されたが,現在ではさまざまな腫瘍においてWT1遺伝子および蛋白の発現が確認されている.しかし,多形腺腫での発現はいまだ明かでない.本研究では多形腺腫におけるWT1蛋白の発現を免疫組織化学的に検索し,腫瘍マーカーとしての有用性を検討した.また,併せてRT-PCRおよびin situ hybridizationにてWT1 mRNAの発現を検索した.免疫組織化学的検索には多形腺腫のホルマリン固定パラフィン包埋組織30例を用い,対象組織として正常唾液腺を使用した.また,多形腺腫8例においてRT-PCRを,さらに3例においてin situ hybridizationを行い,WT1 mRNAの検索を行った.その結果,多形腺腫30例の全例において増殖した腫瘍性筋上皮細胞にWT1蛋白の発現がみられた.RT-PCRでは8例中7例にWT1 mRNAの発現がみられ,in situ hybridizationでは増殖した筋上皮細胞にWT1 mRNAの発現がみられた.したがって,多形腺腫の腫瘍性筋上皮細胞にWT1蛋白およびWT1 mRNAが発現しており,本腫瘍の診断にあたって,抗WT1抗体を用いた免疫組織化学が腫瘍性筋上皮細胞マーカーとして有用であることが示唆された.", "subitem_description_type": "Other"}]}, "item_2_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "Pleomorphic adenoma is the most common type of benign tumor found in salivary glands and is classified as an epithelial tumor. A pathological feature of this tumor is that it consists of two kinds of epithelial cells derived from myoepithelial cells and ductal epithelium. The WT1 gene was originally identified as the gene responsible for Wilms tumor, a type of child renal tumor. The expression of WT1 mRNA and protein has been found in various tumors. However, their expression has not yet been examined in pleomorphic adenoma. In this study, the expression of WT1 protein in pleomorphic adenoma was examined using immunohistochemistry to determine whether it could be used as a marker for neoplastic myoepithelial cells of the pleomorphic adenoma. We also examined where WT1 mRNA was expressed by means of RT-PCR and in situ hybridization. Formalin-fixed, paraffin-embedded tissues from 30 cases of pleomorphic adenoma were prepared for immunohistochemistry, and normal salivary gland tissues were used for the control as a comparison. RT-PCR was conducted on eight cases, and in situ hybridization was also conducted on three cases to examine the expression of WT1 mRNA. We found that the expression of WT1 protein was detected on neoplastic myoepithelial cells of all 30 pleomorphic adenoma cases. RT-PCR showed that WT1 mRNA was expressed in 7 out of the 8 cases, and in situ hybridization revealed that this expression was found specifically in tumoral myoepithelial cells. Together, the results indicate that the WT1 protein and the WT1 mRNA were expressed in tumoral myoepithelial cells of pleomorphic adenoma. This suggests that WT1 can be used as a reliable tumoral marker specific for myoepithelial cells of pleomorphic adenoma.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Oral and Maxillofacial Surgery, Division of Maxillofacial Surgery, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Pathogenesis and Control of Oral Disease, Division of Oral Pathology, School of Dentistry, Iwate Medical University"}]}, "item_2_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "研究"}]}, "item_2_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Original"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔外科学講座顎口腔外科学分野"}, {"subitem_text_value": "岩手医科大学歯学部口腔病因病態制御学講座口腔病理学分野"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2011-05-10"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00007578263.pdf", "filesize": [{"value": "98.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 98500000.0, "url": {"label": "KJ00007578263", "url": "https://iwatemed.repo.nii.ac.jp/record/2006/files/KJ00007578263.pdf"}, "version_id": "3b84b84e-2dbd-40c6-95e3-fbf74a9ef2e1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "WT1", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pleomorphic adenoma", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Salivary gland", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "myoepithelial cell", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "多形腺腫における腫瘍性筋上皮細胞マーカーとしてのWT1の有用性に関する検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "多形腺腫における腫瘍性筋上皮細胞マーカーとしてのWT1の有用性に関する検討"}, {"subitem_title": "Study on WT1 as a neoplastic myoepithelial cell marker for pleomorphic adenoma", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["119", "483"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/2006", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-05-10"}, "publish_date": "2011-05-10", "publish_status": "0", "recid": "2006", "relation": {}, "relation_version_is_last": true, "title": ["多形腺腫における腫瘍性筋上皮細胞マーカーとしてのWT1の有用性に関する検討"], "weko_shared_id": 5}
多形腺腫における腫瘍性筋上皮細胞マーカーとしてのWT1の有用性に関する検討
https://iwatemed.repo.nii.ac.jp/records/2006
https://iwatemed.repo.nii.ac.jp/records/200688c9f1b1-ac38-4ee9-8698-6e54fb288438
名前 / ファイル | ライセンス | アクション |
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KJ00007578263 (98.5 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2011-05-10 | |||||
タイトル | ||||||
タイトル | 多形腺腫における腫瘍性筋上皮細胞マーカーとしてのWT1の有用性に関する検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Study on WT1 as a neoplastic myoepithelial cell marker for pleomorphic adenoma | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
羽田, 朋弘
× 羽田, 朋弘× 三上, 俊成 |
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著者名よみ |
ハダ, トモヒロ
× ハダ, トモヒロ× ミカミ, トシナリ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 36, 号 1, p. 1-18, 発行日 2011-05-10 |