WEKO3
アイテム
{"_buckets": {"deposit": "a48df327-9ce6-4450-b03c-7256ce4216cb"}, "_deposit": {"created_by": 5, "id": "2007", "owners": [5], "pid": {"revision_id": 0, "type": "depid", "value": "2007"}, "status": "published"}, "_oai": {"id": "oai:iwatemed.repo.nii.ac.jp:00002007", "sets": ["119", "483"]}, "author_link": ["7009", "7011", "7012", "7008", "7010", "7007"], "item_2_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-05-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "34", "bibliographicPageStart": "19", "bibliographicVolumeNumber": "36", "bibliographic_titles": [{"bibliographic_title": "岩手医科大学歯学雑誌"}, {"bibliographic_title": "Dental journal of Iwate Medical University", "bibliographic_titleLang": "en"}]}]}, "item_2_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "大橋, 祐生"}], "nameIdentifiers": [{"nameIdentifier": "7007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "藤村, 朗"}], "nameIdentifiers": [{"nameIdentifier": "7008", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_creator_7": {"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "オオハシ, ユウ"}], "nameIdentifiers": [{"nameIdentifier": "7009", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "フジムラ, アキラ"}], "nameIdentifiers": [{"nameIdentifier": "7010", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_creator_8": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OHASHI, Yu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7011", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUJIMURA, Akira", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7012", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "骨欠損に対して再生医学的アプローチをする上で,骨再生組織への新生血管による血液の供給や循環は,安定した骨の形成や感染防御の面において重要であると考えられる.本研究は,bFGF徐放システムによる骨再生モデルを用いて,マイクロフォーカスCTにより同一個体の経時的な骨再生の経過を観察し,連続組織標本を作製することで,骨再生と血管新生の関係を明らかにすることを目的とした.実験方法は,10週齢のWistar系ラットの頭頂骨に直径7mmの骨欠損を形成し,実験群にはbFGF 10μg含有酸性ゼラチンディスクを埋入した.また,対照群には同ディスクに生理食塩液を含浸させたものを埋入した.埋入後2日,1週,2週,4週に同一ラットをマイクロフォーカスCTにて撮影し,三次元画像解析ソフトにて三次元的に骨再生の経過を観察した.また,埋入後1週と4週に連続組織標本をFilm-transfer法にて作製し,H-E染色を行った後,冷却3CCDカメラ装着光学顕微鏡にて二次元コンピューター画像に入力し,再生骨と新生血管の同定を行った.実験の結果,マイクロフォーカスCT所見では,実験群は埋入後2日で再生現象を確認することができなかったが,埋入後1週,2週になると軽度の骨再生が認められ,4週まで継続していた.骨体積計測の結果,埋入後2日,1週,2週では実験群と対照群との間に有意差は認められなかったが,4週には対照群に対し実験群では有意に体積が増加していた(P\u003c0.05).このことから,bFGFによる骨再生誘導は埋入後1週から2週の時期から行われ,4週には骨再生が行われていることが示唆された.組織学的評価では,埋入後1週において,新生血管は,対照群に対し実験群は有意に多く観察された(P\u003c0.001).また,埋入後4週においても実験群は有意に多くの新生血管が観察された(P\u003c0.001).これにより実験群は多数の新生血管により継続的に栄養供給が十分に行われていることが示唆された.以上のことから,骨欠損部へのbFGF含有のAGD埋入によりbFGFが徐放され,血管新生により血液循環と骨断端部への栄養供給が行われ,骨再生誘導に効果を現していることが考えられた.", "subitem_description_type": "Other"}]}, "item_2_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "In devising regenerative medical approaches to bone defects, the blood supply to, and perfusion of, regenerated bone tissue by angiogenesis is considered important to ensure stable bone formation and the prevention of infection. This study was conducted to clarify the relationship between bone regeneration and the angiogenesis bFGF sustained release system by serially observing the process of bone regeneration in the same rats, employing microfocus X-ray CT and preparing serial tissue sections. A bone defect of 7mm in diameter was prepared in the parietal region of 10-week-old Wistar rats, and an acidity gelatin disc (AGD) containing 10μg of bFGF was embedded in the study group. In the control group, the same disc saturated with physiologic saline was embedded. The rats were scanned by microfocus X-ray CT 2 days, 1 week, 2 weeks, and 4 weeks after disc embedding, and the process of bone regeneration was three-dimensionally observed using 3D visualization software. Also, 1 and 4 weeks after disc embedding, serial tissue sections were prepared using the film-transfer method, H-E staining was performed, their 2-dimensional images were input into a computer via an optical microscope with a color-chilled 3CCD camera, and regenerated bone and newly-formed vessels were identified. By microfocus X-ray CT, bone regeneration could not be confirmed 2 days after disc embedding in the study group, but slight bone regeneration was noted 1 and 2 weeks after disc embedding, and bone regeneration continued until after 4 weeks. As a result of measurement of the bone volume, no significant difference was noted between the study and control groups 2 days, 1 week, or 2 weeks after disc embedding, but the bone volume had increased in the study group compared with the control group after 4 weeks (p\u003c0.05). This suggests that bFGF began to induce bone regeneration 1 or 2 weeks after disc embedding and continued to promote bone regeneration until after 4 weeks. Histological evaluation showed that the number of newly-formed vessels was significantly higher in the study group than in the control group 1 week (p\u003c0.001) and 4 weeks (p\u003c0.001) after disc embedding. This suggests that the regenerated bone was sufficiently and continuously supplied blood by many newly formed vessels in the study group. These results suggest that bFGF is slowly released from the AGD containing bFGF embedded at the site of the bone defect, that blood and nutrients are supplied by angiogenesis, and that the treatment is effective for inducing bone regeneration.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Oral and Maxillofacial Surgery, Division of Oral Surgery, School of Dentistry, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Oral Biology, Division of Oral Anatomy, School of Dentistry, Iwate Medical University"}]}, "item_2_text_2": {"attribute_name": "記事種別(日)", "attribute_value_mlt": [{"subitem_text_value": "研究"}]}, "item_2_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Original"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学歯学部口腔外科学講座歯科口腔外科学分野"}, {"subitem_text_value": "岩手医科大学歯学部口腔機能構造学講座口腔解剖学分野"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2011-05-10"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00007578264.pdf", "filesize": [{"value": "27.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 27700000.0, "url": {"label": "KJ00007578264", "url": "https://iwatemed.repo.nii.ac.jp/record/2007/files/KJ00007578264.pdf"}, "version_id": "b2e6c1b1-61a8-45ad-a518-13cda8052c56"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "basic fibroblast growth factor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "bone regeneration", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "angiogenesis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "microfocus X-ray CT", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "acidity gelatin disc", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討"}, {"subitem_title": "A study of bone regeneration and angiogenesis using bFGF sustained release system", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["119", "483"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/2007", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-05-10"}, "publish_date": "2011-05-10", "publish_status": "0", "recid": "2007", "relation": {}, "relation_version_is_last": true, "title": ["bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討"], "weko_shared_id": 5}
bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討
https://iwatemed.repo.nii.ac.jp/records/2007
https://iwatemed.repo.nii.ac.jp/records/2007be55ec0c-3d75-4399-af4e-b496d11d045f
名前 / ファイル | ライセンス | アクション |
---|---|---|
KJ00007578264 (27.7 MB)
|
Creative Commons : 表示 - 非営利 - 改変禁止
|
Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2011-05-10 | |||||
タイトル | ||||||
タイトル | bFGF徐放性材料を用いた骨再生モデルにおける骨再生と血管新生の検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A study of bone regeneration and angiogenesis using bFGF sustained release system | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
大橋, 祐生
× 大橋, 祐生× 藤村, 朗 |
|||||
著者名よみ |
オオハシ, ユウ
× オオハシ, ユウ× フジムラ, アキラ |
|||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 36, 号 1, p. 19-34, 発行日 2011-05-10 |