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It has been mentioned that the acquisition of the nucleotides from the host cells is probably mediated through their own nucleases. However, in Mycoplasma salivarium, the existence of nuclease has not yet been known. In this study, we examined the nuclease activity in M. salivarium, and analyzed the properties of the enzyme. The Triton X-114 solubilized supernatant (Tx) obtained from lysate of M. salivarium was used for the enzymatic analysis. The nuclease activity was detected as a specific band of 25kDa by SDS-PAGE and the following in-gel digestion (SDS-PAGE nuclease assay). The nuclease activity of Tx was strictly dependent on Ca^\u003c2+\u003e. The nuclease was heat-stable, and the optimum pH was in the range of 7-9. The nuclease showed a low substrate specificity. From these findings, it was revealed that M. salivarium has a novel type of 25kDa and Ca^\u003c2+\u003e dependent nuclease. Moreover, we examined whether Tx could cleave the chromatin DNA in the nuclei originated from eukaryote cells. Consequently, Tx cleaved the DNA of HS-72 B cell nuclei in vitro. 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Mycoplasma salivariumにおけるヌクレアーゼの存在とその特性
https://iwatemed.repo.nii.ac.jp/records/1581
https://iwatemed.repo.nii.ac.jp/records/1581eeacf9f3-f598-4dc3-b5a8-fca200f6183b
名前 / ファイル | ライセンス | アクション |
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KJ00005130301 (1.1 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-12-19 | |||||
タイトル | ||||||
タイトル | Mycoplasma salivariumにおけるヌクレアーゼの存在とその特性 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Existence of the nuclease in Mycoplasma salivarium and its characterization | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
小川, 恵子
× 小川, 恵子× 加茂, 政晴× 水城, 春美× 客本, 斉子 |
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著者名よみ |
オガワ, ケイコ
× オガワ, ケイコ× カモ, マサハル× ミズキ, ハルミ× キャクモト, セイコ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 33, 号 3, p. 109-122, 発行日 2008-12-19 |